Dr. Pablo Perez-Pinera has developed a method to divide prime editing tools into smaller components that are small enough to be delivered in adeno-associated viruses, an...
Dr. Pablo Perez-Pinera has developed a method to divide prime editing tools into smaller components that are small enough to be delivered in adeno-associated viruses, an FDA-approved vehicle for therapeutic use. Prime editors have recently shown great promise as gene editing tools with greater specificity and fewer off-target effects than existing CRISPR-Cas based methods. This technology has potential as a research tool as well as a therapeutic strategy.
Dr. Jeffrey Moore along with colleagues at Northwestern University has developed new nonproteinogenic substrates for use in a system that uses genetic reprogramming to...
Dr. Jeffrey Moore along with colleagues at Northwestern University has developed new nonproteinogenic substrates for use in a system that uses genetic reprogramming to create polymers. The system allows translation of polymers using substrates not used for protein synthesis. Presently over 200 substrates have been discovered, Dr. Moore has found over 40 new substrates. This technology allows for the creation of polypeptides and sequence-defined polymers in vitro and in vivo.
Dr. Thomas Gaj has developed a novel prime editor based on a Staphylococcus aureus Cas9 ortholog. Furthermore, he has developed split intein versions of the prime editing...
Dr. Thomas Gaj has developed a novel prime editor based on a Staphylococcus aureus Cas9 ortholog. Furthermore, he has developed split intein versions of the prime editing tools that are small enough to be dual-delivered in adeno-associated viruses, an FDA-approved vehicle for therapeutic use. This invention has been shown to improve targeting capability of the prime editors by several fold. Prime editors have recently shown great promise as gene editing tools with greater specificity and fewer off-target effects than existing CRISPR-Cas based methods. This technology has potential as a research tool as well as a therapeutic strategy.
Dr. Aksimentiev has developed a new solid-state nanopore DNA sequencing method that affords superior control over DNA translocation. By creating custom-sized nanopores and...
Dr. Aksimentiev has developed a new solid-state nanopore DNA sequencing method that affords superior control over DNA translocation. By creating custom-sized nanopores and exercising specifically designed microfluidic flow, this method allows for "flossing" of a DNA or RNA strand between nanopores. This allows for multiple reads and improved accuracy of sequencing strands. In comparison to existing platforms, this technology offers superior control over the passage of DNA through the nanopore and improves the accuracy of nanopore sequencing results. This technology can be integrated with most existing nanopore sequencing platforms. In addition to DNA, this technology has potential utility for characterizing other biopolymers, including proteins.
