Researchers from the University of Illinois have developed a highly sensitive, graphene field effect transistor (gFET) that can detect DNA in samples. This technology uses...
Researchers from the University of Illinois have developed a highly sensitive, graphene field effect transistor (gFET) that can detect DNA in samples. This technology uses Loop-Mediated Isothermal Amplification (LAMP) and crumpled graphene in order to detect the presence of a target segment of DNA within a provided sample. By using crumpled graphene, gFET, and LAMP Dr. Ganguli's technology is able rapidly detect pathogens using entirely electronic means bringing diagnostics directly to point of care medical practice, all while being less expensive, more sensitive, and quicker than other traditional, gold-standard pathogen diagnostics methods. This technology is even able to detect molecular concentrations down to 8 zeptomolar.
Publication: Ganguli, A., Faramarzi, V., Mostafa, A., Hwang, M. T., You, S., & Bashir, R. (2020). High Sensitivity Graphene Field Effect Transistor-Based Detection of DNA Amplification. Advanced Functional Materials, 30(28), [2001031]. https://doi.org/10.1002/adfm.202001031
Professor Brian Cunningham's research group at the University of Illinois has created a technology for ultrasensitive detection of gold nanoparticles, allowing different...
Professor Brian Cunningham's research group at the University of Illinois has created a technology for ultrasensitive detection of gold nanoparticles, allowing different assays and applications.
These single step, isothermal, room temperature, one-pot assays can process a sample volume as low as ~20 ul. These assays are performed with an inexpensive, small device based on a low intensity LED (no laser), that allows for simple data quantification by counting (no fancy imaging sensor needed) and multiplexing by sample splitting.
Applications of this technology include:
Activate Capture and Digital Counting (AC+DC) for detection of miRNA, based on gold nanoparticles tags prepared with DNA toehold probes (DNA-AuNPs). (LOD = 100 aM, 2h; no purification and amplification required)
Activate Capture and Digital Counting (AC+DC) for detection of proteins, based on secondary antibody-functionalized gold nanoparticles (2oAb-AuNPs) within 15 min (LOD = 10pg/ml) (pictured below)
Targeting Recycling Amplification Process (TRAP) for miRNA detection, based on strand displacement reactions (LOD = 0.1aM, 20 min)
Activate Cleve and Count (ACC) for ctDNA detection based on gold nanoparticles released after target recognition by CRISPR/Cas (LOD = 1zM, 60 min) (pictured below
Researchers from the University of Illinois have developed a COVID-19 testing system named SPOT that consists of a rapid, highly sensitive and accurate assay and a battery...
Researchers from the University of Illinois have developed a COVID-19 testing system named SPOT that consists of a rapid, highly sensitive and accurate assay and a battery-powered portable device. The assay combines RT-LAMP with an Argonaute protein from hyperthermophilic archaeon Pyrococcus furiosus for a precise recognition and cleavage of target DNA in saliva samples. The simplicity of the SPOT system enables rapid diagnosis of COVID-19 without a trained personnel and minimal sample handling.
Researchers from the University of Illinois have developed a low-cost, reusable sensor for the detection of hydrogen peroxide. The sensor detects H2O2 over the range of...
Researchers from the University of Illinois have developed a low-cost, reusable sensor for the detection of hydrogen peroxide. The sensor detects H2O2 over the range of biologically relevant concentrations (including as low as 0.016 uM) with sensitivity thresholds of up to 4903 uA mM-1 cm-2, with performance demonstrated in test solutions designed to mimic human blood. The device uses Bi2Te3 as its active material and can be fabricated at room temperature using conventional processes, making the sensors far less expensive than alternative commercial or pre-commercial solutions.
RT-PCR RNA based approaches, the most popular method for SARS-CoV-2 detection, are limited by large numbers of consumables and reagents required, the need for well-...
RT-PCR RNA based approaches, the most popular method for SARS-CoV-2 detection, are limited by large numbers of consumables and reagents required, the need for well-optimized protocols, challenges with mutations, and RNA lingering after infectivity. Researchers at the University of Illinois, led by Dr. Aaron Timperman, have developed a protein-based rapid, reliable and inexpensive nanofluidic/microfluidic system to detect viral proteins with limits of detection at clinically relevant levels. The system receives a sample, purifies the virions, prepares the sample and sprays the sample directly into a mass spectrometry. This protein-based approach allows the collection of additional information such sequence mutations, modifications that affect protein function, and bound antibodies.
Prof. Yurii Vlasov and Christopher Kenji Brenden from the University of Illinois have designed an electrochemical biosensor capable of detecting small concentrations of...
Prof. Yurii Vlasov and Christopher Kenji Brenden from the University of Illinois have designed an electrochemical biosensor capable of detecting small concentrations of neurochemicals in vivo with high spatiotemporal resolution.
The probe design include a thin-layer design with microfluidic channels to decrease probe size and improve the voltage-current response from electroactive biomolecules. Electrodes are placed within the microfluidic channels of the cell to prevent degradation of electrode surfaces by immune responses and attack by biological agents.
Valve-free flow switching allows for in situ regeneration and calibration of the probe electrodes without removing the probe from the target biological tissue. This allows for longer measurements periods without experience large drift in electrochemical signals.
