Dr. Boppart has created a new manner of calibrating multimodal optical instruments. Unlike prior solutions, such as fluorescent beads, this invention does not bleach and can be used calibrate instruments across multiple frames longitudinally. This solution can also be used to normalize imaging data and is able to calibrate instruments for 2D and 3D multimodal instruments so that standardized imaging under realistic, tissue-sample-like settings can be achieved.
