Three-Dimensional Nonlinear Interferometric Vibrational Imaging of Molecules

Nonlinear Interferometric Vibrational Imaging (NIVI) NIVI offers unmatched capabilities in biomedical imaging by performing non-invasive three-dimensional molecular imaging of living specimens and tissue. This imaging platform interferometrically detects nonlinear optical signals based on the vibrational states of atomic bonds within target molecules and may be used for diagnostics and for delivery of focused ablative treatment. 

Developed as part of a portfolio of optical contrast agents and molecular detection technologies that enhance the ability of optical coherence tomography (OCT) to non-invasively map molecules in living specimens and diagnose disease where it starts. OCT utilizes low-coherence interferometry to measure the intensity of reflected or backscattered light to form images with micrometer resolution, is readily integrated with existing optical instrumentation and has application across a wide range of biological, medical, surgical, and non-biological specialties.


  • Images a range of molecular species simultaneously with a single instrument
  • Requires no exogenous labels to detect specific molecules
  • Permits precise density determination without background signals
  • Allows 3-D discrimination of molecular density and enhancement

Provides 2D and 3D imaging of biological tissues, showing both molecular information and structural information. These measurements could provide clinical diagnostic value without requiring a biopsy. These innovations offer better sensitivity, better false signal rejection, and more flexibility than CARS microscopes, and can potentially be much cheaper and easier to operate. 

A set of CARS (Coherent Anti-stokes Raman Spectroscopy) related inventions that efficiently enable in vivo three-dimensional imaging of biological tissues without added stains or markers. The features examined are actually the density of molecules in tissue with particular molecular ro-vibrational features. This is a major improvement over NIVI (Non-linear Interferometric Vibrational Imaging).

Determination of both structural and chemical attributes of biological tissues either as biopsy or in vivo.

Laboratory set-ups exist, many animal and human tissues have been imaged. Ready for product development engineering to: reduce cost, provide customer-centric packaging, documentation, regulatory compliance, etc.