Integrated System for Genome Modification in E. coli


Dr. Kuhlman from the University of Illinois at Urbana-Champaign has created a new system that provides a single editing tool for a variety of genome modifications. This method has the advantage over CRISPR-Cas9 system in not requiring prior modification of the genome and requires only locus-specific primers for genome editing.

This technology is an optimized Landing Pad system that can use positive and negative selection to select for insertion, deletion, and knockout events at genomic targets using specific primers contain homology region A, B, and C. This approach provides a powerful tool for manipulating the genome.


  • The Landing-Pad system can integrate large fragments of DNA, can be targeted anywhere in the genome and ab be applicable to deletions and knockouts as well.
  • Unlike the CRISPR-Cas9 system, this technique does not require a special promoter for gene expression.
  • The Landing-Pad system can target different loci with only locus-specific primer design.