All of These
Medical professionals need techniques that will help them diagnose diseases before debilitating symptoms begin to manifest in patients. Point of care testing, or...
Medical professionals need techniques that will help them diagnose diseases before debilitating symptoms begin to manifest in patients. Point of care testing, or bedside testing, enables rapid diagnostic tests to be performed at a patient's bedside.
Dr. Bashir's lab has invented a label-free, electronic method for quantifying various biomarkers from blood. This method may be incorporated into a portable handheld device which enables healthcare professionals to perform complete blood count diagnostics at the point of patient care. Key advantages over existing competing technology include versatility, scalability, and decreased cost of production.
Prof. Oelze from the University of Illinois has developed a novel technique of processing ultrasound images which will improve the spatial resolution by a factor...
Prof. Oelze from the University of Illinois has developed a novel technique of processing ultrasound images which will improve the spatial resolution by a factor of 6.9 (at least) over the diffraction limited approaches. It will also provide significant noise reduction.
Dr. Boppart from the University of Illinois has developed new computational algorithms to improve Optical Coherence Tomography (OCT) imaging. This will provide...
Dr. Boppart from the University of Illinois has developed new computational algorithms to improve Optical Coherence Tomography (OCT) imaging. This will provide surgeons with a better view of cancerous tissue and allow improved treatment of numerous diseases.
Dr. Aksimentiev from the University of Illinois and researchers at TU Delft in The Netherlands have developed a plasmonic nanopore technology for DNA sequencing....
Dr. Aksimentiev from the University of Illinois and researchers at TU Delft in The Netherlands have developed a plasmonic nanopore technology for DNA sequencing. One of the hurdles facing current nanopore-based DNA sequencing technologies is the inability to control DNA speeds passing through the pore. The group has developed nano-antennas that can focus high intensity light on the nanopore to create a "hot-spot" to trap and slow down DNA movement, which can help read long lengths of DNA more efficiently.
CT scanners are gathering more data than ever, far exceeding the ability of the hardware and software to process and analyze the data and consequently slowing...
CT scanners are gathering more data than ever, far exceeding the ability of the hardware and software to process and analyze the data and consequently slowing down diagnosis. This is becoming a more serious issue as the field moves from fan-beam (2-D and spiral) to cone-beam (fast volumetric or 3-D) acquisition. These algorithms were developed to address this problem. This suite of patented and patent-pending algorithms reconstructs tomographic images for standard (i.e., 2-D) and volumetric (i.e., 3-D) CT scans 10 to 100 times faster than conventional methods for typical image sizes, lowering scanning costs, increasing throughput, enabling improved image quality, and freeing up precious computer resources.
Fast Hierarchical Backprojection Method for Imaging
This method involves backprojecting a sinogram to a tomographic image by subdividing it into subsinograms corresponding to subimages as small as a single pixel. The subsinograms are backprojected to produce corresponding subimages, and the subimages then are aggregated to create the full tomographic image. As with several of the algorithms described above, speed is greatly enhanced through the use of an approximate decomposition algorithm.
Fast Hierarchical Backprojection for 3-D Radon Transform
With this method, data from a 3-D sinogram are backprojected to form a 3-D volume. An input sinogram is subdivided into subsinograms, which are further subdivided until they represent volumes as small as a single voxel. The subvolumes then are aggregated to form a final volume. Again, this algorithm combines an accurate but slow subdivision algorithm with a faster but less accurate subdivision algorithm, reaching an accurate result quickly.
Fast Hierarchical Native Fan-Beam Tomographic Reconstruction Algorithms
This family of native divergent beam algorithms can be used to reconstruct all divergent-beam tomographic data, including single- and multi-slice 2-D fan-beam and 3-D cone-beam with arbitrary scan trajectories, including single circle and spiral trajectories for short and long objects. The algorithms operate directly on the data without prior rebinning to parallel beam projections. Both reprojection and backprojection functions are available.
Multilevel Domain Decomposition Method for Fast Reprojection of Images
The method involves decomposing an image into one or more subimages, reprojecting the subimages into sinograms (i.e., arrays of numbers), scaling the sinograms, and aggregating the subimage sinograms into a single sinogram of the entire image.
Fast Hierarchical Reprojection Algorithm for Tomography
This variation on the above reprojection method combines an exact algorithm, which is accurate but slow, with an approximation algorithm, which is less accurate but fast, to create an accurate result in a short time.
Fast Hierarchical Reprojection Algorithm for 3-D Radon Transforms
This algorithm is based on 3-D radon transform, which is a mathematical model used in volumetric imaging. It begins by dividing the 3-D image into subvolumes as small as a single voxel. These subvolumes then are reprojected at various orientations to form subsinograms. The subsinograms are then successively aggregated and processed to form a full sinogram for the initial volume. Like the previous algorithm, this technology combines a highly accurate slow subdivision algorithm with a faster but less accurate subdivision algorithm to quickly obtain an accurate result.
Qualified companies are invited to license the algorithms as well as enter into agreements that will allow evaluation and suitable modifications to the algorithms that may be necessary for use in specific applications.
Industrial Imaging: By reconstructing tomograms faster than do previous methods, these algorithms dramatically increase the number of items that can be scanned per hour (i.e., throughput), eliminating the "image reconstruction bottleneck" and significantly reducing manufacturing/ inspections costs. These algorithms can be used with any industry inspection using CT scans:
Security Imaging: The faster imaging speeds enabled by these algorithms will offer dramatic improvements in 3-D CT inspection of baggage or containers for the detection of weapons, explosives, or other hazardous materials. This will be a tremendous benefit as U.S. airports strive to meet new federal baggage inspection requirements.
Researchers and practitioners in university, industrial and national labs increasingly rely on low temperatures in the development and study of products. For...
Researchers and practitioners in university, industrial and national labs increasingly rely on low temperatures in the development and study of products. For example, pharmaceutical companies routinely use low temperatures to study disease targets and markers.
The biomolecules are always in buffers. Since the pH of buffers changes dramatically upon lowering temperature, the biolmolecules are not the same pH when they are at the room temperature. The change of pH can dramatically change the structural and functional properties of the biomolecules so that information obtained at low temperature does not reflect its properties at room temperature or physiological temperature. Additionally, since the exact pH buffer at different temperatures is not often predictable, the results obtained using a temperature variable pH buffer cannot easily correlate back to the pH at room temperature.
This invention is a new design of pH buffers that show negligible pH change upon cooling to low temperatures, including cryotempatures. This invention solves the problem of significant change in apparent pH of a glycerol solution of common biological buffers upon cooling to cryotemperature. By combining a buffer that increases pH upon cooling with one that decreases pH upon cooling, the apparent pH change upon cooling to cryotemperature is minimized.
Advanced molecular imaging tools combined with investigative tools like biochemical and cell based assays have thepotential to unravel complex molecular processes...
Advanced molecular imaging tools combined with investigative tools like biochemical and cell based assays have thepotential to unravel complex molecular processes. These tools combined with high throughput screening can significantly impact diagnostics for cancer screening and accelerate drug discovery. These inventions are two new Fluorescence Resonance Energy Transfer (FRET) biosensor pairs, one composed of two new colors, mOrange2 and mCherry and the other composed of a modified high-sensitive ECFP/YPet pair that can significantly enhance the dynamic range of a variety of biosensors. The ECFP/YPet can detect signaling events with high spatio-temporal resolutions which makes it an ideal readout indicator for high throughput screening.
FRET technology and genetically encoded FRET biosensors are very useful in detecting active molecular events inlive cells with high temporal and spatial resolutions. FRET occurs when two flurophores are in proximity with the emission spectrum of the donoroverlapping with the excitation spectrum of the acceptor. To date, the most popular FRET pair is cyan and yellow fluorescent proteins (CFP and YFP).
The new FRET biosensor pairs mOrange2/mCherry are proteins with different colors and spectrally distinctive from the CFP/YFP pair. This invention opens up the possibility of lighting two diagnostic biomarkers in the same cell e.g., cancer cells, thus providing a double criterion high-fidelity assay to differentiate cancer vs. normal cells.
To provide proof-of-concept that mOrange2 and mCherry are suitable as novel FRET biosensors, they were operably linked to the protein recognition sequence of MMP-MT1. MMP-MT1 is an enzyme belonging to the matrix metalloproteinase family that has known roles in cancer metastasis. The basis of this assay is that when MT1-MMP is inactive, the mCherry and mOrange2 and positioned in proximity and favor a strong FRET between the two moieties. Indeed, in-vitro assays established that the mOrange/mCherry pair can serve as a reliable and sensitive indicator of the status of MT1-MMP activation and can potentially be applied to other biomarker assays.
The ECFP/YPet pair provides a high-sensitive biosensor for the visualization of molecular hierarchy at different subcellular locations inlive cells. In-vitro assays revealedthat the ECFP/YPet pair exhibits significantly enhanced dynamic range of the MT1-MMP biosensor compared to currently available CFP/YFP FRET pairs. When quantified, the ECFP/YPet pair showed a 570% change (% change in basal level upon stimulation of MT1-MMP) when compared to only 90-100% in existing FRET pairs. The ECFP/YPet pair has also been successfully applied to other classes of proteins, like kinases, that are important therapeutic targets in human cancer.
These and other genetically engineered biosensors can serve as a research tool to monitor different signaling cascades in live mammalian cells with high sensitivity. The developed MT1-MMP biosensor and potentially other protein biosensors can provide a powerful tool for the spatiotemporal imaging of protein functions in cancer development e.g. detection of circulating tumor cells (CTCs). Furthermore, these biosensors can serve as an excellent high-throughput reporting system forthe detection of cancer and the development of inhibitors for cancer therapeutics.
The novel mOrange2/mCherry pair presents a method to simultaneously visualize two active signaling events in the same cell when combined with existing FRET pairsThe ECFP/YPet pair can serve as a high-sensitive biosensor with significantly enhanced dynamic ranges compared to existing CFP/YFP FRET pairs. The ECFP/Ypet pair can be operably linked to any protein recognition sequence to detect activity of that protein in live cells. The pair also includes a positively charged tag which allows 100% efficiency ofdetection. This invention demonstrates an ECFP/Ypet pair operably linked to MT1-MMP, a tumor metastasis biomarker, to accurately detect circulating tumor cells in blood samples.
A system that incorporates teachings of the present disclosure may include, for example, an ionizing radiation sensor having a first scintillator for generating...
A system that incorporates teachings of the present disclosure may include, for example, an ionizing radiation sensor having a first scintillator for generating photons from incoming ionizing radiation, an imaging intensifier for amplifying the photons, and an electron-multiplying charge-coupled device (EMCCD) coupled to the imaging intensifier for sensing the amplified photons generated by the imaging intensifier.
A radio frequency (RF) coil comprising a plurality of electrically uninterrupted conductive legs, each leg having a first end and a second end, and at least one...
A radio frequency (RF) coil comprising a plurality of electrically uninterrupted conductive legs, each leg having a first end and a second end, and at least one continuous conductor electrically connected to the first ends of the legs. Frequency tuning of the coil is achieved by translating, along the legs, an electrically continuous tuning band that includes a capacitor closed about the axis of the coil in proximity to the conductive legs. Maintaining electrical symmetry of the coil results in tuning ranges of at least 30 percent of the nominal value of the resonant frequency.