Harmonic Optical Tomography of Nonlinear Structures

A collaborative research effort between Professor Gabriel Popescu and co-inventors from Colorado State University has resulted in a new imaging technique, Harmonic Optical Tomography (HOT). HOT is a 3D imaging technique facilitating the visualization of tissues and other biological samples on a microscopic scale.

This technique is based on using holographic information, which measures light patterns, providing unique capability of 3D imaging of non symmetric structures. Compared to second harmonic generation microscopy, HOT is less vulnerable to vibrations and unwanted microscopic drifts leading to sharper images, increased reproducibility and better quantitative interpretation of images.

a, A 3D view of the wide-field SHG intensity measurement of a murine muscle slice. b, A 3D view of the sample tissue block from a after HOT reconstruction.