This technology adapts conventional optical microscopes for visualizable detection and sensing in nanometric volumes. Conversion is fast and low cost, and the resulting...
This technology adapts conventional optical microscopes for visualizable detection and sensing in nanometric volumes. Conversion is fast and low cost, and the resulting device is easy to operate, label-free, and non-destructive.
Applications
Microscopy, semiconductor wafer inspection, characterizing physical, biological, chemical properties of materials, bio-sensing
Dr. Bhargava has developed a new method and device for imaging with a discrete frequency infrared spectroscopic microscope. Vibrational circular dichroism (VCD) is useful...
Dr. Bhargava has developed a new method and device for imaging with a discrete frequency infrared spectroscopic microscope. Vibrational circular dichroism (VCD) is useful for quantifying chirality, and this method allows for VCD imaging and chirality mapping of solid samples. VCD imaging measurements can examine small and localized changes in tissue and assist in quantization and separation of chirality.
A collaborative research effort between Professor Gabriel Popescu and co-inventors from Colorado State University has resulted in a new imaging technique, Harmonic Optical...
A collaborative research effort between Professor Gabriel Popescu and co-inventors from Colorado State University has resulted in a new imaging technique, Harmonic Optical Tomography (HOT). HOT is a 3D imaging technique facilitating the visualization of tissues and other biological samples on a microscopic scale.
This technique is based on using holographic information, which measures light patterns, providing unique capability of 3D imaging of non symmetric structures. Compared to second harmonic generation microscopy, HOT is less vulnerable to vibrations and unwanted microscopic drifts leading to sharper images, increased reproducibility and better quantitative interpretation of images.
a, A 3D view of the wide-field SHG intensity measurement of a murine muscle slice. b, A 3D view of the sample tissue block from a after HOT reconstruction.
Professor Stephen Boppart and researchers from the Beckman Institute for Advanced Science and Technology have developed a method and apparatus for capturing four...
Professor Stephen Boppart and researchers from the Beckman Institute for Advanced Science and Technology have developed a method and apparatus for capturing four phase shifted interference patterns for instantaneous phase sensitive optical coherence tomography. This technique is a label free imaging technique with phase, polarization and spectroscopic sensitivity, while using incoherent illumination and capturing phase-shifted images in a single camera. It can be used as a commercial imaging system for imaging biomedical and biological samples, specifically for imaging cellular dynamics. In addition, the technique can also be used as a non-invasive diagnostic tool to assess and monitor diseases that alter optically accessible tissues (e.g. melanoma, skin diseases. This technique improves light-use efficiency, robustness and increases information content that can be extracted from images.
Benefit
Phase sensitive optical coherence tomographyis an imaging technique with phase, polarization and spectroscopic sensitivity, while using incoherent illumination and capturing phase-shifted images in single camera.
Phase sensitive optical coherence tomography improves light-use efficiency, robustness and increases information content that can be extracted from images.
Market Application
This technique can be used as a commercial imaging system for imaging biomedical and biological samples, such as cells or tissues.
Figure 1. Examples of imaging applications. Images a and c (insets zoomed in b and d) show an average amplitude, while b and c show: Dynamic OCM image calculated as variance of sequence of 50 phase images
Dr. Boppart has created a new manner of calibrating multimodal optical instruments. Unlike prior solutions, such as fluorescent beads, this invention does not bleach and...
Dr. Boppart has created a new manner of calibrating multimodal optical instruments. Unlike prior solutions, such as fluorescent beads, this invention does not bleach and can be used calibrate instruments across multiple frames longitudinally. This solution can also be used to normalize imaging data and is able to calibrate instruments for 2D and 3D multimodal instruments so that standardized imaging under realistic, tissue-sample-like settings can be achieved.
