Technologies

Chromatography

Counter-current chromatography (CCC) is a high resolution separation technique useful for fractionating complex mixtures, such as natural product extracts, under very mild conditions based on their relative solubility in two immiscible phases. Cross-platform comparison of CCC chromatograms is essential for the calibration, quality control, trouble shooting, and test performance of instruments, as well as rapid development of new CCC/CPC (Counter-partition chromatography) columns.
High speed countercurrent chromatography (HSCCC) and centrifugal partition chromatography (CPC) are the major techniques that are used for the separation of a large variety of natural products. Proper selection of a biphasic solvent system for counter-chromatography (CCC) separations is very important because in CCC, the solvent acts as both the column as well as the eluant. But the lack of a clear method for comparing the many possible biphasic solvent systems or solvent system family performance seems to be a major impediment in more widespread use of these techniques.
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